In vivo evaluation of wound healing properties of superoxidized solution (SOS) using an excision wound model

Superoxidized solution (SOS), which is made by electrolysi of sodium chloride, is a nontoxic disinfectant used to eradicate broad spectrum of microorganism and does not seem to damage host tissue or facilitate bacterial resistance. In addition, it is well tolerated, improves granulation of wound tissue, and reduces the need of debridement and less pain reported during the treatment. The purpose of this study was to assess wound healing effect of SOS and gel of SOS. Excision wounds were induced in Swiss Webster mice. Animals were assigned into five groups: negative control group received no treatment; positive control group received a wound ointment (Sebamed cream); SOS group; SOS gel group and blank gel group treated with gel base. Wounds were observed macroscopically until they were fully epithelialized, and they were sampled every three days to be examined microscopically. Efficiency of treatment for each animal in proportion to its three days earlier, revealed that repair of SOS and positive control group were accelerated significantly in day nine in comparison with day six. While it happened three days later for SOS gel group. According to microscopically observation, epithelialization occurred three days earlier in SOS and SOS gel group. It might be concluded that SOS and SOS gel group accelerate healing through inducing epithelialization. Result of investigation effect of gender on wound treatment revealed no significant differences between male and female, although the type of treatment can affect the wound healing activity for each gender.